Research Institute for Biotechnology and Environment, Nong Lam University, HCMC, Vietnam
Title of the talk: Loop Mediated Isothermal Amplification (LAMP) for rapid detection of microbial pathogens: potential applications in Vietnam
Until now, many worldwide scientists have developed numerous techniques for the detection of microbial pathogens, in particularly the use of PCR and realtime PCR based methods, RFLP analysis, ELISA, sequencing and etc. However, those methods are normally labour and time intensive and can only be undertaken in well-equipped labs. Therefore, an alternative approach known as Loop Mediated Isothermal amplification (LAMP) has been studied and developed so much in the world as a rapid, simple and low cost diagnostic tool for microbial pathogens within one hour of sampling in the field or on-site detection that is appropriate to the poor and developing country. Herein, we developed LAMP based assays for rapid detection of many groups of phytoplamas causing witches’ broom (WB) and white leaf (WL) diseases in longan, cassava and sugercane respectively. Moreover, in-field detection of such diseases was also performed to indicate their advantages of LAMP method in comparison with other conventional techniques. Another applications of LAMP assays were also developed for the detection of various Salmonella serovars known as causal agent of enteric or typhoid fever in human; Burkholderia pseudomallei causing Melioidosis so-called Whitmore disease and Hepatitus B virus in human. The results in such studies will be helpful for practical application of LAMP based diagnostics for the detection and control of diseases in plant, animal and human as well as for the development of new and promising diagnostic system for on-site detection of bacterial pathogens in Vietnam.
Nguyen Bao Quoc has completed his PhD in the field of biological and environmental sciences at Kobe University, Japan in 2008 and then spent 4 years as JSPS postdoctoral fellow and research associate in Kobe University, Japan and Dartmouth College in USA. He is now a lecturer at the Research Institute for Biotechnology and Environment, Nong Lam University, HCMC, Vietnam. He has published more than 10 papers in reputed journals, book chapter and has been serving as reviewers for many international and national journals.