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Dr. Nguyen Thi Tuyet Nhung
Institute of Biotechnology, Vietnam Academy of Science and Technology, Hanoi, Vietnam.
Title of the talk:Lactobacillus plantarum IBT16 isolated from Vietnamese fermented shrimp lowers serum triglyceride level and alters intestinal bacteria in diet-induced obese C57/BL6 mice
The prevalence of obesity is increasing steadily among adults, adolescents and children and is now considered a worldwide epidemic. The causes driving the obesity include environmental, genetic, neural and endocrine factors. More recently obesity was associated with a specific profile of the bacterial gut microbiota. Some probiotics can beneficially affect the host by improving the balance of commensal intestinal microflora. Among the key members of probiotic group, Lactobacillus plantarum is a flexible and versatile organism that inhabits a wide array of environmental niches including the human gastrointestinal tract. The present study determined the effect of oral administration of L.plantarum IBT16 isolated from Vietnamese fermented shrimp on lipid metabolism and intestinal microflora in diet-induced obese C57/BL6 mice. Mice that had been administered Western diet with 108 CFU of live L.plantarum IBT16 showed significant reduction in the serum triglyceride level. Furthermore, mRNA expression of lipid metabolism regulating PPAR alpha gene in adipose tissue in mice administered L. plantarum IBT16 was significantly higher whereas fatty acid synthase gene was significantly lower than those in control mice. Furthermore, the administration of L. plantarum IBT16 restored some bacterial strains which reduced in C57/BL6 mice fed the Western diet. In conclusion, this novel class of probiotic lactobacilli with triglyceride-lowering activity in C57/BL6 mice merits further investigation to treat obese patients.
I received my Ph.D from Institut National de la Recherche Scientifique (INRS), Quebec University, Canada in 2014, under the supervision of Professor Alain Fournier. During my PhD, the major focus of my research is the isolation, characterization of insulinotropic peptides from snake venom for type 2 diabetes treatment. Based on the sequences of natural insulinotropic peptides, we did de novo design, synthesis and structure activity studies of shorter peptide fragments that are synthesized by solid phase peptide synthesis following Fmoc – chemistry. From 2015 to 2016, I worked as a postdoc researcher with Dr. Masuko Kobori at the National Food Research Institute, Tsukuba, Japan. In Japan, my main work was investigation of (1) the effect of Lactobacillus plantarum on diet-induced obese mice and (2) effect of 20 amino acid peptides on GLUT4 translocation in skeletal muscle cells, liver cells and adipocytes. I am now working in the Molecular Microbiology Laboratory at the Institute of Biotechnology, Vietnam Academy of Science and Technology. My main concerns are focusing on studying Lactobacilli and Bifidobacterium bacteria derived from intestinal microbes of vietnamses healthy adults to support and treatment of obesity-related diseases. I have published 2 papers in journals of Medicinal Chemistry, Chembiochem, and many other national papers. I got funded for 2 projects.