Department of Biotechnology, Nong Lam University, Vietnam
Title of the talk: Pantropic PRRSV Replicon Containing Vsv Glycoprotein As A Vaccine Candidate
Porcine reproductive and respiratory virus (PRRSV) is a devastating virus in swine and is difficult to grow in cell culture partially due to a limited number of cell lines susceptible and permissive for it. Using glycoprotein G gene (GVSV) from a pantropic virus, vesicular stomatitis virus (VSV), to replace genes encoding the minor glycoproteins of PRRSV in FL12, a putative infectious clone of American serotype PRRSV, the resultant chimeric virus was recovered (FL12GΔ2-4). FL12GΔ2-4 can infect and complete its viral life cycle in different cell lines which originally unsusceptible to PRRSV such as BHK-21, PK-15, HEK-293, HeLa… The spreading of the virus was confirmed by immunofluorescent method and its expression of GVSV and PRRSV proteins was shown by Western blot and neutralization assay. Further investigation indicate that the chimeric virus is stable genetically and the pantropic effect of GVSV can also be observed when G protein is expressed from a separate DNA plasmid. Overall, our studies indicate that GVSV can be incorporated into PRRSV virion irrespective of being provided in cis or in trans and the incorporation confers the virus to be able to infect and spread in multiple cell lines.
Dr. Phat is presently a lecturer and a researcher at Nong Lam University Ho Chi Minh City, Vietnam (formerly named University of Agriculture and Forestry). With the experiences about molecular techniques and viral biology obtained during his doctoral and postdoctoral programs in the University of Nebraska – Lincoln (NE, USA), he is now also serving as a consultant for clinical/molecular diagnosis of veterinary diseases and research for vaccine development in an international Veterinary Diagnostic Laboratory in Ho Chi Minh City of Vietnam. He has 13 peer-reviewed papers published in reputed journals.