Institute of Genetics, University of Bayreuth, Germany
Title of the talk: Optimization of intracellular recombinant protein production in bacteria
The production of recombinant proteins in microbial systems has revolutionized biotechnology. The gene of interest is cloned into an expression vector, transformed into the host of choice followed by production of the recombinant protein and its subsequent purification. Since no expression system produces high amounts of any recombinant protein, different parameters have to be optimized. I will discuss the following aspects: plasmid type, promoter system, production of the recombinant protein with a removable purification/solubility tag, translation signals, codon usage, inclusion body formation, disulfide bond formation, chaperone co-expression, and genome reduction. At the end, I will compare the bacterial systems Escherichia coli, Bacillus subtilis, Lactobacillus and Corynebacterium.
Wolfgang Schumann has completed his Ph.D. at the age of 27 years from the Mainz University and did postdoctoral studies at the Universities of Goettingen and Konstanz. Since 1986, he is Professor at the University of Bayreuth and since October 2013 Distinguished Professor of the VNUHCM-University of Science, Ho Chi Minh City, Vietnam. He has published more than 150 papers in reputed journals related bacterial gene expression and function. In addition, he has been serving as an editorial board member of two scientific journals.